The table below lists the volumes of cell culture required for Miniprep, Midiprep, and Maxiprep plasmid DNA purification depending on the plasmid copy number: If you are using a low copy number plasmid, you will need to use a higher volume of cell culture, as directed in the protocol. High copy number plasmids typically yield >2 µg DNA/ml LB whereas low-copy-number plasmids yield <2 µg DNA/ml LB. If possible, use a high-copy-number plasmid to obtain a good yield of plasmid DNA with a small volume of culture. The PureLink™ HiPure Plasmid DNA Purification Kits allow purification of all types and sizes of plasmid DNA, including BAC, bacmids, and ssM13 DNAs.
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